Bodipy 493/503 thermo

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August undefined, 2024

WebOct 1, 2024 · Lipid drop (LD) accumulation was evaluated using BODIPY 493/503 (10 μg/mL; Thermo Fisher, D2148) staining. The nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) staining solution. The average LD intensity from BODIPY 493/503 staining was quantified using Image J software (National Institutes of Health). Web工作时间. 周一至周五：9:00-18:00. 咨询电话. 400-686-0611. 在线咨询. qq咨询 qq咨询刘家河高元明

Bodipy 493 503 Thermo Fisher Bioz

WebApr 12, 2024 · Cellular lipid droplets were stained with 0.38 ÂµM BODIPY 493/503 (ThermoFisher Scientific) for 25 min. Nuclei of the cells were stained with 0.38 ÂµM DAPI (Roche) for 20 min. Microscopy was performed using a Leica SP8 confocal microscope (Leica Microsystems Inc.) with spectral detection and a Leica HC FLUOTAR 25x/NA 0.95 … WebJun 28, 2024 · BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. 2 μM BODIPY … karl hess anarchist cookbook

Figure 3 Use of BODIPY (493/503) to Visualize Intramuscular …

WebStaining with BODIPY 493/503 (D-3922) has been shown by flow cytometry to be more specific for cellular lipid droplets than staining with nile red.19Mark Cooper at University of Washington has found that BODIPY 505/515 (D-3921) rap- idly permeates cell membranes of live zebrafish embryos, selec- tively staining cytoplasmic yolk platelets. WebSep 5, 2016 · BODIPY 493/503 dye (Thermo Fisher Scientific, Waltham, MA,USA ) was used for staining the neutral lipid droplets (LDs), and the staining method was performed according to the manufacturer and... WebSep 5, 2016 · BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry. Lipid droplets (LDs) are ubiquitous, dynamic … laws about black powder guns in california

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Oligo Configuration Options Thermo Fisher Scientific - PG

WebBODIPY™ 493/503 dye is bright, green fluorescent dye with similar excitation and emission to fluorescein (FITC) or Alexa Fluor™ 488 dye. It has a high extinction … WebTriglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal … karl helo attorney at law lafayette laWebStaining of LDs with BODIPY (493/503) in cross section of rat tibialis anterior (TA) muscle demonstrates that LDs are not limited to muscle fibers expressing slow MHC. (a) Fibers stained with BODIPY (green) demonstrate the appearance of spherical LDs within the muscle fibers as previously described with Oil Red O staining procedures. karl h fendt wage and salary division

"WebFluorescent Bodipy 493 503 Dye Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. … " - Bodipy 493/503 thermo

Bodipy 493/503 thermo

Product Information Revised: 15-July-2003

WebBODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells. 1, 2 BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. WebMar 22, 2024 · For tumor cells cocultured with adipocytes, cancer cells were labeled with BODIPY® 493/503 (to stain neutral lipids) at 2.5 ng/mL, with rhodamine-phalloidin (to stain actin cytoskeleton)...

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WebDescription With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. We recommend dissolving in high-quality anhydrous DMSO to attain a stock concentration of 1 to 10 mM. Order Info Shipping Condition: Room Temperature WebJul 1, 2024 · 艾美捷 Lumiprobe BDP 493/503 是一种用于脂质标记的绿色荧光硼二吡咯亚甲基染料。. 它用于显微镜下的细胞膜可视化，以及疏水介质的染色和追踪。. 艾美捷 Lumiprobe BDP 493/503脂质染色剂化学性质：. 形态：红色至橙色晶体. 分子量： 262.11. CAS号： 121207-31-6. 分子式： C ...

WebWith its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar … With its nonpolar structure and long-wavelength absorption and fluorescence, BO… TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebDescription With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and …

WebThermo Scientific CAT: E6779-1ML BODIPY™ 493/503 staining Thermo Scientific CAT: D3922 Lipofectamine 2000 Thermo Scientific CAT: 11668019 Pierce™ BCA Protein Assay Kit Thermo Scientific CAT: 23227 MG132 Selleck CAT: S2619 Chloroquine diphosphate Selleck CAT: S4157 Triglyceride (TG) Nanjing Jiancheng Bioengineering Institute CAT: … WebEx/Em = 493/503. BODIPY 558/568 C 12: Stock aliquots in DMSO (-30°C freezer) at 2.12 mM. Working solutions range around 10-80 uM but you should experiment to determine the best staining for your sample. Ex/Em = approx 558/568; emits into red region. BODIPY FL C5-ceramide complexed to BSA: Reconstitute 5 mg unit in 150 µM sterile distilled water.

WebThermo Fisher lipid probe bodipy 493 503 Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99 lipid probe bodipy 493 503 - by Bioz Stars , 2024-03 86 / 100 stars Images bodipy 493 503 lipid probes ( Thermo Fisher ) Thermo Fisher is a verified supplier Thermo Fisher manufactures this product About News Press Release Team

Webtraditional neutral lipid stains such as Invitrogen ™ BODIPY 493/503 dye or Nile Red, and are available with green, red, and deep red fluor - escence emission. Learn more about our LDL probes Thermo Fisher Scientific offers the most complete portfolio of unlabeled and fluorescently labeled native LDL, OxLDL, and AcLDL for the study karl hill unleashedWebJun 1, 2016 · Excess lipid is stored in intracellular organelles known as lipid droplets. This unit discusses techniques for the visualization of lipid droplets and associated proteins in cultured mammalian cells. Protocols for the detection of lipid droplets in fixed or live cells with BODIPY 493/503 are included. The best method for combining visualization ... karl hess gmbh \u0026 co. kg burbachWebCustomer Review. BODIPY 493/503 (Pyrromethene 546) is a fluorescent neutral lipid dye (Ex/Em: 493/503 nm). BODIPY 493/503 can facilitate quantification of neutral lipid … karl henry water bottleWebBODIPY™ 493/503 具有非极性结构、吸收波长较长且发荧光，可作为中性脂质染色剂及油和其他非极性脂质的示踪剂。 For Research Use Only. Not for use in diagnostic … karl hess: anarchism in americaWebFeb 27, 2010 · BODIPY 493/503 fluorescence by successive B and G excitations. Huh7 cells were fixed and stained with BODIPY 493/503 and DAPI. The three figures (a–c) were taken in the same field … laws about briberyWebOct 31, 2001 · We have prepared a series of bovine serum albumins (BSA) that have been site-selectively labeled at cysteine-34 with one of four different sulfhydryl-selective boron dipyrromethene difluoride (BODIPY) fluorescent probes (BODIPY FL IA, BODIPY FL C 1 IA, BODIPY 530/550 IA, and BODIPY 493/503 MB). We determine how the choice of … karl hosterman state college paWebThermo Fisher lipid probe bodipy 493 503 Average 86 stars, based on 1 article reviews Price from $9.99 to $1999.99 lipid probe bodipy 493 503 - by Bioz Stars , 2024-03 86 / … karl holding chris\u0027s baby