Cytc antibody
WebAug 3, 2013 · Furthermore SUG increase CytC, AIF, Smac/Diablo and CASP-3 protein expression in cells in culture. ... Barcelona, Spain). Monoclonal anti-glial fibrillary acidic protein (anti-GFAP) antibody (1:500) was from Sigma-Aldrich Biotech (Madrid, Spain). Monoclonal anti-MAP-2 protein (anti-MAP-2) antibody (1:500) and monoclonal anti … WebFeb 1, 2016 · Samples and human CytC standards were diluted in Calibrator Diluent and incubated with microtiter wells pre-coated with a monoclonal antibody specific for human CytC. After washing away unbound proteins, wells were further incubated with an HRP-linked monoclonal antibody anti-human CytC, color developed with …
Cytc antibody
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WebJan 24, 2012 · Anti KdgM and anti CytC antibodies were detected with anti IgG labelled with Alexa fluor 488 (green labelling) and DNA was stained with DAPI (blue labelling). … WebMonoclonal Antibody for studying cytochrome C. Cited in 300 publications. Validated for Western Blotting, Immunohistochemistry (Paraffin). …
WebCytochrome c is a 12-15 kDa electron transporting protein located in the inner mitochondrial membrane. Upon apoptotic stimulation, … WebDec 17, 2015 · Caspases regulate cell apoptosis. CytC release activates caspase-3 and AIF is a caspase-independent mitochondrial death effector. Consistent with prominent increases in activated cleavage product of caspase-3, mitochondrial CytC and AIF were released into the cytosol and nucleus in the vehicle-treated group, respectively.
WebCaspase-9 activities were determined using the double antibody sandwich method of ELISA. Each column represents the mean ± SEM of three independent experiments. Different lowercase letters indicate the ... (including cytosolic CytC, cytosolic AIF, mitochondrial CytC, mitochondrial AIF, Mfn2 and FIS1), signaling pathways mediated by … WebCytochrome c is a component of the electron transport chain in mitochondria and it accepts electrons from the complex III and transfers electrons to the complex IV. In Arabidopsis, …
WebThe obtained results indicated that the mt-nAChRs targeted by anti-mitochondrial antibodies produced by PV patients are coupled to inhibition of CytC release, and that nicotinergic stimulation can abolish PVIgG-dependent activation of intrinsic apoptosis in …
WebSep 21, 2009 · Slides were further incubated with monoclonal anti-cyto-chrome c (CytC) antibody (BD Biosciences; 1:200 in PBST containing 5 mg/ml BSA; 2 h, room temperature) followed by Alexa Fluor 488-conjugated anti-mouse IgG (Invitrogen; 1:200 in PBST with 5 mg/ml BSA; 1 h, room temperature). Slides were mounted and images were acquired … tank vision frcWebCytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from … tank vests from wwiiWebMar 26, 1998 · The structural mechanism involved in the binding of the IgG1 monoclonal antibody E8 to its highly charged protein antigen horse cytochrome c (cyt c) is revealed … tank vision incWebCytochrome c belongs to class I of the c-type cytochrome family and contains a characteristic CXXCH (cysteine-any-any-cysteine-histidine) amino acid motif that binds heme. This motif is located towards the N … tank vixens charactersWebAqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol. This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only. Store at -20°C, for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. tank verses tank combat scenesWebA positive result for cyclic citrullinated peptide (CCP) antibodies may be suggestive of rheumatoid arthritis (RA) if compatible clinical features of disease are present. … tank vehicle testWebAntibodies that detect Cytochrome C can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry … tank volume calculations gallons