Hucct1 rbe hccc-9810 cclp-1

Author: piwu

August undefined, 2024

WebCCK-8 assay showed that NNT-AS1 knockdown suppressed the proliferation ability of CCA cells (CCLP1, QBC-939) compared with control group ( Figure 2A ).. In accordance with prolifera- Web对于两个最常见的FGFR2::BICC1融合蛋白，通过四聚体交换实验，一组合成的肽被成功地交换成HLA-A02:01和HLA-A24:02四聚体。此外，我们还生成了iCCA细胞株HuCCT1和HCCC-9810，它们异位表达FGFR2:BICC1融合蛋白。 MHC-I免疫肽直接检测FGFR2:BICC1过表达的HuCCT1细胞的融合肽LTLTTNEIME。这些结果支持了融合多肽可以由相应的HLA等 …

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Webwith 1 μM bortezomib for 0, 3, 6 hours. (F) Immunoblots of lysates generated from RBE cells transfected with negative control (NC) or sgRNA targeting PTEN and exposed to 1 μM bortezomib for 6 hours. (G-I) Immunoblots of lysates generated from RBE, HCCC-9810, QBC939, SK-CHA-1, CCLP1, and MZ-CHA-1 treated with 5 μg/ml tunicamycin (TM). Web23 sep. 2024 · ( D) Cell survival curves for RBE, HuCCT1, and HCCC-9810 cells treated the indicated 20 S proteasome inhibitors with a dose escalation from 0 to 100 μM. Data are presented as mean ± SD ( n = 3) from one of two independent experiments. ( E) Cell survival of CCA cell lines exposed to serial dilutions of bortezomib for 30 hours. hka perth

CCLP-1人胆管细胞 - ChemicalBook

WebThe human biliary epithelial cell line HIBEC and four human CCA cell lines (TFK-1, CCLP, HCCC-9810, and HUCCT1) were acquired from the Shanghai Institute of Cell Biology (Shanghai, China) and cultivated in RPMI-1640 medium (HyClone, USA) containing 10% fetal bovine serum (HyClone) and 1% penicillin/streptomycin (Gibco, USA) in a … Web14 jan. 2024 · HCCC-9810 and TFK1 cells have low endogenous expression of these two miRNAs, and high endogenous expression was detected in HuCCT1 and RBE cells (Figures 1C, D). FIGURE 1 Figure 1 Expression of miR-144-5p and miR-451a in cholangiocarcinoma specimens and cell lines. Web1、细胞复苏. 1）配制完全培养基：基础培养基+胎牛血清+双抗（特殊培养基特殊配置）；. 2）细胞复苏：取5ml完全培养基于15ml离心管中，37℃水浴锅预热，从液氮管（或者-80度冰箱）中快速取出冻存的细胞，放入37℃水浴锅中，摇晃使快速化冻（1min左右 ... falken azenis rt-615k treadwear

Hucct1 rbe hccc-9810 cclp-1

Hccc 9810 Bioz Ratings For Life-Science Research

WebYuHuang,1,2 YangKong,1,2 LufeiZhang ,1,2 TianyuHe,1,2 XiaohuZhou,1,2 ... Hccc-cellswerepur-chased from the Chinese Academy of Sciences Shanghai BranchCellBank(Shanghai,China).eHuccT-cellline was maintained at our institution. e Hccc- cell line was cultured in Roswell Park Memorial Institute (RPMI) Web其中,HC toxin对CCLP-1,HuCCT1, TFK-1,RBE的半数有效抑制率,分别为297.6+80.4 nM、520.0+43.0 nM, ... 3.HC toxin对CCLP-1细胞活力既有抑制作用也有促进作用,抑制作用大于促进作用,促进作用可能与HC toxin激活Notch信号通路有关,可通过联合Notch通路阻滞剂抵消HC toxin对CCLP-1 ...

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WebThe SNP profiles of HCCC-9810 and Calu-6 also revealed that they were different cell lines. 150 cell lines with unique profiles demonstrated ... PC-9, Anglne and CCLP-1. According to the original descrip-tion, we divided the remaining cell lines (264 cell lines) into two categories: the Chinese model (cell lines established in China, n = 71 ... Web结果 HUCCT1、HCCC-9810、RBE细胞系中miR-590水平分别为0.37±0.084、0.31±0.071和0.53±0.089，显著低于人正常肝内胆管上皮细胞系HIBEC的1.12±0.201，差异具有统计学意义（ P ＜0.05）。 miR-590可抑制野生型SIP1 3’UTR报告基因载体的荧光素酶活性，而对突变型SIP1 3’UTR-MUT的荧光素酶活性无影响。转染miR-590 mimics可以下调HUCCT1 …

http://www.biofeng.com/xibao/xibaozhu/HUCC-T1.html Web28 feb. 2024 · Methods: ICC cell lines (CCLP-1, RBE, and HCCC-9810) were treated with metformin and/or ATO; the anti-proliferation effect was evaluated by cell viability, cell …

Web24 aug. 2024 · HUCCT1，RBE，HCCC-9810，CCLP-1. 常用动物模型：胆管癌移植瘤小鼠模型. 15. 胰腺癌常用细胞系（株）： AsPC-1，BxPC-3，Capan-1，Capan-2，CFPAC … Web10 dec. 2024 · Recent study showed that 1 688 780 new cancer cases and 600 920 cancer deaths occur in the United States during 2024.1 What was worse, these numbers are growing constantly due to lack of effective therapy for cancer.2 Therefore, further research should be launched to detect new therapeutic biomarker for cancer treatment.

WebHuCCT1, RBE, CCLP-1 and HCCC-9810 CCA cell lines, and human intrahepatic biliary epithelial cells (HIBECs) were obtained from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences. Cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) ...

Web细胞描述： hccc-9810细胞株源自一位女性肝胆管细胞癌患者。hccc-9810细胞呈上皮细胞样，染色体模式数为71，但染色体数目可以在22-117的范围内变动。倍增时间为20.4小时。AFP，CEA和CA19-9的分泌水平低，在裸鼠中的成瘤率为20%。细胞特性： 1）来源：人，女性；肝胆管细胞癌 2） h ka photo dil meWeb18 jul. 2024 · HCCC-9810, CCLP1 and RBE cells were obtained from the Cell Bank of Type Culture of Chinese Academy of Sciences (Shanghai, People’s Republic of China). The other CCA cells including QBC939, HuCCT1, KMBC and human intrahepatic biliary epithelial cells (HIBEC) were preserved in our laboratory. hkaprWeb18 jul. 2024 · HCCC-9810, CCLP1 and RBE cells were obtained from the Cell Bank of Type Culture of Chinese Academy of Sciences (Shanghai, People’s Republic of China). The … falken azenis rt660 245/40/18Webb、加1 mL消化液（0.25%Trypsin-0.02% EDTA）于培养瓶中，使消化液浸润所有细胞，将培养瓶置于37℃培养箱中消化1-3 min（视细胞情况而定），然后在显微镜下观察细胞消化情况，若细胞大部分变圆并脱落，迅速拿回操作台，轻敲几下培养瓶后加2-3ml完全培养基终止消 … hkapmcWebHUCC-T1人肝胆管癌细胞接受后处理 1) 收到细胞后，请检查是否漏液，如果漏液，请拍照片发给我们。 2) 请先在显微镜下确认细胞生长状态，去掉封口膜并将T25瓶置于37℃培养约2-3h。 3) 弃去T25瓶中的培养基，添加 6ml本公司附带的完全培养基。 4) 如果细胞密度达80%-90%请及时进行细胞传代，传代培养用6ml本公司附带的完全培养基。 5) 接到细胞 … falken azenis rt660 215/45/15Webhccc-9810细胞源自一位女性肝胆管细胞癌患者，呈上皮细胞样。 HCCC-9810细胞染色体模式数为71，但其染色体数目可以在22-117的范围内变动。 HCCC-9810细胞倍增时间 … falken azenis rt660 mileageWeb9 nov. 2024 · Moreover, wound healing and transwell assays showed that the migration abilities of HCCC-9810 and RBE cell lines were significantly reduced upon EIF3H inhibition (Figure 2C,D). Flow cytometry analysis of apoptotic cells indicated a considerably greater percentage of apoptotic cells in EIF3H-depleted HCCC-9810 and RBE cells (Figure 2E). hkap modul orbis