WebbphlF encoding PhlF repressor from Pseudomonas protegens Pf-5. PhlF gave 83-fold repression of GFP expression from PphlF ( BBa_K1725000 ), whereas the control, TetR ( … WebbPhlF repressor repressing pPhlfp promoter. There will be no Cre recombinase produced here. Upon an induction, cI inhibits pcI in its dimer form. There will be Cre recombinase production here. 2 System of Reactions. We can describe the Sensing module as elaborated above with this set of reactions:

WikiGenes - phlF - transcriptional repressor PhlF

WebbTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map WebbSupplemental Figure 1. Repressor expression plasmids. Both constitutive (top two panels) and inducible expression (bottom panel) vectors are illustrated for each repressor. In the case of the constitutively expressed repressors, expression is controlled by the hEF1a promoter, whereas inducible expression is mediated by either the TRE-tight ... share outlook contacts with android phone

Tuning dCas9

Webbing it to the PhlF repressor (dCas9* PhlF). Both the 30 bp PhlF operator and 20 bp sgRNA binding site are required to repress a promoter. The larger re-gion required for … Webb13 juni 2016 · A P phlF promoter, which is repressed by PhlF ( 25 ), reports repressor activity via the expression of RFP (Supplementary Figure S6). Trigger factor was chosen … WebbThe repression by PhlF is due to its interaction with an inverted repeated sequence, phO, located downstream of the phlA transcriptional start site. Cross-linking experiments … share outlook folders